Session VI : November 21, 2024
Alternative EM Sample Preparations
Program for Session VI
Miriam Lucas
Xavier Heiligenstein
​One for All, All for One: A Close Look at In-Resin Fluorescence Protocols for CLEM
Miriam Lucas is a staff scientist and lecturer at the Scientific Center for Optical and Electron Microscopy (ScopeM) at ETH Zurich. She graduated in Environmental Engineering from the University of Applied Sciences in Hamburg Germany, and received a PhD from the University of Hamburg, working in close cooperation with the R&D Microscopy Department of Beiersdorf AG, on the description of the pigmentation of human skin by means of correlative light and electron microscopy. Since 2007 she is implementing techniques for correlative light and electron microscopy (CLEM) at ScopeM, with a focus on advancing biological imaging into the third dimension. This includes the establishment of preparation protocols suitable for multimodal imaging from LM to vEM and TEM8, as well as image processing for modeling and 3D illustration.
Xavier Heiligenstein obtained a master’s degree in 'Cell Biology, Physiology and Pathology' at the university Paris 7 - Denis Diderot in 2007. In 2011, he completed his PhD at the EMBL, Heidelberg, in 'Cell Biology and Biophysics'. During his PhD, he invented the CryoCapsule1,2, a vehicle to conduct Live-HPF CLEM studies. During his post-doctoral work at Institut Curie (2012-2018), UMR144, CNRS, he invented several technologies for CLEM: the HPM Live µCliquez ou appuyez ici pour entrer du texte., eC-CLEMCliquez ou appuyez ici pour entrer du texte., the R221 resinin parallel to launching his own company, CryoCapCell. Since 2018, he is the chief scientific officer of CryoCapCell where he continues developing and commercializing innovative technologies for CLEM..
Jared A. Lapkovsky
Introduction to Low Voltage Transmission Electron Microscopy for Biological Applications
In his role as International Sales Manager at Delong America, Jared Lapkovsky demonstrates a profound expertise in the applications of Low Voltage Electron Microscopy (LVEM), leveraging his 15 years of experience. With a deep understanding of the technology's capabilities, Jared adeptly communicates the benefits of LVEM to clients across diverse industries, including materials and life sciences, as well as clinical applications. His strategic guidance and customer-centric approach enable organizations to harness the power of LVEM for advanced imaging and analysis, revolutionizing their research and development processes. Through Jared’s leadership, Delong America continues to be at the forefront of delivering cutting-edge electron microscopy solutions to a global clientele, driving forward scientific discovery and technological advancement.
Lucille A. Giannuzzi
Cryo ex situ lift out manipulation of cryo FIB prepared TEM Specimens
Lucille is President of EXpressLO LLC in Lehigh Acres, FL, President of L.A. Giannuzzi & Associates LLC and Regional Sales Manager, TESCAN USA. She has nearly 30 years in focused ion beam (FIB) research, development, and specimen manipulation experience, particularly for transmission electron microscopy (TEM) applications. She is a Fellow of AVS, MSA, and MAS and co-editor of the book “Introduction to Focused Ion Beams.”
Thomas Edwards
The NCI’s National Cryo-EM Facility: Breaking Down Barriers for Cryo-EM Access
Thomas Edwards earned degrees in History and Chemistry from Michigan State University. He obtained his PhD in Biological Sciences from Purdue University studying virus induced cellular membrane structures that occur during flavivirus and alphavirus infections. He has been with NCEF since its founding in 2017 and was elevated to facility manager in 2021.