Session 1 : January 19, 2023
Serial Block Face-SEM
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Melainia McClain
Melainia has a degree in Biology from University of Missouri-Columbia. She has been in the field of electron microscopy for over 15 years and is our expert for all EM related instrumentation, especially the 3View serial block face SEM system. She is passionate about all aspects of 3D electron microscopy, and is always up for learning new electron microscopy techniques!
Shiliang Steven Zhang
Shiliang Steven Zhang, Ph.D. is the Manager of the Confocal and Electron Microscopy Core at IRP/NIDA/NIH/DHHS in Baltimore, MD since 2015. He holds a M.S. in Applied Statistics from Syracuse University and a Ph.D. in Molecular Biology from State University of New York, Syracuse, NY. From 2005 to 2009 he was a Postdoctoral Fellow in the Department of Molecular Biology and Genetics at Johns Hopkins University School of Medicine in Baltimore. From 2009 to 2015 he was a Research Fellow at Neuronal Networks Section, National Institute on Drug Abuse also in Baltimore.
Trace Christensen
Trace is a Research and Development Specialist at the Mayo Clinic Microscopy and Cell Analysis Core Facility in Rochester, MN. He received a B.S. degree in Biology from Saint John’s University and M.S. degree in Biotechnology Enterprise from John’s Hopkin’s University. Trace has also spent time training at MBL, CU Boulder, and the Scripps Research Institute. He has worked in biological microscopy and research for over thirty years at Mayo and is involved in designing experiments and developing protocols to facilitate the research goals of investigators using a variety of imaging modalities. He has extensive experience in microwave processing and 3DEM techniques. Recently he has contributed significantly to work in mitochondrial dynamics of Alzheimer’s disease, diabetes, Usher syndrome, and ischemic strokes. Trace enjoys mentoring both undergrad and graduate students. He has helped design and build a 3DEM service at Mayo that includes processing, serial block-face imaging, and data analysis - utilized by researchers at Mayo and throughout the country. Trace has been an active member of MSA since 2003 and served as Treasurer of the Diagnostic & Biomedical Microscopy focused interest group for several years. He is also a member of the Minnesota Microscopy Society and is actively working to help bring new microscopy and data analysis technologies to local universities and research institutions.
Flash Talks
Batch staining TEM grids with the Modified Hiraoka Grid Staining Kit
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Philip Seifert
EM Specialist & Morphology Core Manager, Morphology Core
Mass General Brigham, Schepens Eye Research Institute of Mass Eye and Ear
Boston MA
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Automated specimen prep of <1x1x1 mm tissue specimens for vEM
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Robotics Engineer
Heartland BioTech
Madison, WI