Session III : May 18, 2023
Array Tomography & Serial Sectioning
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Jemima Burden & Ian White
SEM Array Tomography is perhaps the simplest, cheapest and most flexible of the volume EM techniques, and thus makes the ideal entry point for those wishing to extend their ultrastructural imaging into the 3rd dimension. Whilst initially it can still seem quite daunting, results can be achieved with mostly standard EM lab equipment by even newcomers to volume EM.
Jemima is Head of Electron Microscopy at the Laboratory for Molecular Cell Biology (LMCB) at University College London (UCL). She received her BSc from University of Bath and her PhD from Imperial College London, where her fascination for microscopy began, before specialising in electron microscopy at the LMCB.
Ian is Deputy Head of Electron Microscopy at the LMCB. Having completed his PhD at the University of Leeds and a postdoctoral position at the University of Sheffield, Ian moved to UCL where he began to learn the techniques involved in biological electron microscopy, before joining the LMCB as a specialist.
Jemima and Ian support the needs of researchers at the LMCB and beyond in their EM research on cells, tissues and model organisms, focussing on the most technically demanding CLEM, and 3D EM projects. In 2020 the LMCB EM unit branched out into Scanning Electron Microscopy (SEM), specifically array tomography, to extend their ability to image large areas in X Y and Z, and have been developing methods to make this more routinely possible and accessible for all EM users.
Réza Shahidi
Réza Shahidi completed his PhD while working in the lab of Professor Gáspár Jékely at Max Planck Institute for Developmental Biology in Tübingen Germany. He moved to the University of Exeter's Living Systems Institute in the UK, where he continued as a Postdoctoral Research Fellow focusing on the development and mapping of animal nervous system networks using volume electron microscopy. He uses serial section electron microscopy and related approaches to create large, high-resolution 3D image datasets from which to reconstruct the cellular complement of an organism, including its neurons and their synaptic connections. Subsequently, he has returned to Germany to assume the co-headship of the Electron Microscopy Core Facility with Dr. Charlotta Funaya at the University of Heidelberg. Reza is also an associate fellow of the Higher Education Academy and a member of the Royal Microscopy Society. He has been involved in teaching techniques in electron microscopy for many years. A more recent example is the EMBO practical course in advance electron microscopy techniques.
Jenna Schardt
Jenna is a Research Associate III at the Allen Institute for Brain Science. She has spent the past 6.5 years working with Stephen J Smith automating, and refining Array Tomography. She holds a Masters in Materials Science with an emphasis in electron microscopy.
Tech Talk
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Array Tomography Training and Network – a CZI-funded initiative
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Martina Schifferer
Electron Microscopy Hub
DZNE - German Center for Neurodegenerative Diseases
Munich, Germany
Tech Talk
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Low-cost Solution for Array Tomography Microtome Manipulators
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Michael Moore
OSCAR Imaging Facility
Delaware State University